Journal: Journal of advanced research

Article Title: SAP deficiency aggravates periodontitis possibly via C5a-C5aR signaling-mediated defective macrophage phagocytosis of Porphyromonas gingivalis.

doi: 10.1016/j.jare.2022.10.003

Figure Lengend Snippet: Fig. 2. SAP deficiency aggravates periodontitis and inflammation. (a) Micro-CT 3D reconstruction images of the maxilla of wildtype and SAP-KO mice. (b) CEJ-ABC distance was analyzed from Micro-CT images (n = 5). (c) Histological images of H&E and TRAP staining, and representative CD34 and CD45 immunohistochemistry images of the periodontium in wildtype and SAP-KO periodontitis mice. Quantitative analysis of CEJ-ABC distance (d) and osteoclasts (e) from histological images (n = 5). Quantitative analysis of CD34 (f) and CD45 (g) positive cells from immunohistochemistry images (n = 3). (h-j) Inflammatory cytokine mRNA expression in the periodontium of wildtype and SAP-KO periodontitis mice (n = 3). (k-m) Protein level expression of inflammatory cytokines detected by ELISA (n = 5). Red dot line, CEJ level; Black dot line, ABC level; Blue double arrow line, CEJ-ABC distance. Yellow ‘‘R”, tooth root; Yellow ‘‘B”, alveolar bone. Black arrow, osteoclasts. Data are presented as mean ± SD. The significant difference among the groups, *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The protein level of SAP in the human gingival lysate was analyzed using the human SAP ELISA kit (Cusabio, Wuhan, China) and in mouse periodontal tissue lysate, liver lysate, and serum was analyzed using the mouse SAP ELISA kit (SEB539Mu, Cloud-Clone Corp, Wuhan, China).

Techniques: Micro-CT, Staining, Immunohistochemistry, Expressing, Enzyme-linked Immunosorbent Assay